ELISA/assay

Rat Prolactin ELISA Kit

MBS727546

96 Strip Wells

675 USD

ELISA Kit

Rat Prolactin ELISA Kit

Prolactin

Prolactin; Prolactin; prolactin; decidual prolactin; prolactin

PRL

PRL; PRL; PRL

PRL_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This PRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PRL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Principle of the Assay: PRL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PRL. Standards or samples are then added to the microtiter plate wells and PRL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PRL present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PRL are added to each well to "sandwich" the PRL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PRL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRL concentration in each sample is interpolated from this standard curve.

130930

P01236.1

25,876 Da

Amyloids Pathway 366238!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Disease Pathway 530764!!ErbB4 Signaling Events Pathway 137991!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!Growth Hormone Receptor Signaling Pathway 477128!!Immune System Pathway 106386!!Jak-STAT Signaling Pathway 83077

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

96 Strip Wells

675 USD