ELISA/assay

Human Leptin ELISA Kit

MBS727499

48-Strip-Wells

440 USD

ELISA Kit

Human Leptin ELISA Kit

[Leptin]

[leptin; leptin; obesity homolog; leptin<]

[Leptin]

[lep; ob; xlep]

Human

This assay has high sensitivity and excellent specificity for detection of Leptin-Ab. No significant cross-reactivity or interference between Leptin-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Leptin-Ab and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 250- 5000pg/mL. Sensitivity: 1.0pg/mL

Signal Transduction

Intended Uses: This Leptin-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Leptin-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: Leptin-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a Leptin antigen and an Leptin-Ab-HRP conjugate. The assay sample and buffer are incubated together with Leptin-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Leptin-Ab concentration since Leptin-Ab from samples and Leptin-Ab-HRP conjugate compete for the Leptin antigen binding site. Since the number of sites is limited, as more sites are occupied by Leptin-Ab from the sample, fewer sites are left to bind Leptin-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin-Ab concentration in each sample is interpolated from this standard curve.

148233267

NP_001089183.1

NM_001095714.1

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48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415