ELISA/assay

Mouse Calprotectin ELISA Kit

MBS727460

48-Strip-Wells

470 USD

ELISA Kit

Mouse Calprotectin ELISA Kit

Calprotectin

calprotectin=urinary stone protein band A {N-terminal}, partial

CP

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Immunology

Intended Uses: This CP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CP antibody and an CP-HRP conjugate. The assay sample and buffer are incubated together with CP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CP concentration since CP from samples and CP-HRP conjugate compete for the anti-CP antibody binding site. Since the number of sites is limited, as more sites are occupied by CP from the sample, fewer sites are left to bind CP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CP concentration in each sample is interpolated from this standard curve.

806925

AAB33355.1

48-Strip-Wells

470 USD

96-Strip-Wells

675