Mouse Dickkopf Related Protein 2 ELISA Kit | MyBioSource MBS727436 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Dickkopf Related Protein 2 ELISA Kit

catalog :

MBS727436

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS727436

products type :

ELISA Kit

products full name :

Mouse Dickkopf Related Protein 2 ELISA Kit

products short name :

Dickkopf Related Protein 2

other names :

dickkopf-related protein 2; Dickkopf-related protein 2; dickkopf-related protein 2; hDkk-2; dickkopf-2; dickkopf 2 homolog; dickkopf homolog 2; dickkopf related protein-2; dickkopf WNT signaling pathway inhibitor 2

products gene name :

DKK2

other gene names :

DKK2; DKK2; DKK-2; UNQ682/PRO1316; Dickkopf-2; Dkk-2; hDkk-2

uniprot entry name :

DKK2_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of Dikk2. No significant cross-reactivity or interference between Dikk2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Dikk2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This Dikk2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Dikk2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: Dikk2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Dikk2 antibody and an Dikk2-HRP conjugate. The assay sample and buffer are incubated together with Dikk2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Dikk2 concentration since Dikk2 from samples and Dikk2-HRP conjugate compete for the anti-Dikk2 antibody binding site. Since the number of sites is limited, as more sites are occupied by Dikk2 from the sample, fewer sites are left to bind Dikk2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Dikk2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

7657023

ncbi acc num :

NP_055236.1

ncbi gb acc num :

NM_014421.2

ncbi mol weight :

28,447 Da

ncbi pathways :

Disease Pathway (530764); Presenilin Action In Notch And Wnt Signaling Pathway (137914); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1016209); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1016191); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (980475); Wnt Signaling Pathway (83061); Wnt Signaling Pathway (471); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1016210)

ncbi summary :

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008]

uniprot summary :

DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family. Protein type: Secreted; Inhibitor; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q25. Cellular Component: extracellular space. Biological Process: Wnt receptor signaling pathway; multicellular organismal development

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage