ELISA/assay

Human Cofilin 2, Muscle ELISA Kit

MBS727433

48-Strip-Wells

470 USD

ELISA Kit

Human Cofilin 2, Muscle ELISA Kit

Cofilin 2, Muscle

cofilin-2 isoform 1; Cofilin-2; cofilin-2; cofilin, muscle isoform; nemaline myopathy type 7; cofilin 2 (muscle); Cofilin, muscle isoform

CFL2

CFL2; CFL2; NEM7

COF2_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CFL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CFL-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CFL-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CFL-2 antibody and an CFL-2-HRP conjugate. The assay sample and buffer are incubated together with CFL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CFL-2 concentration since CFL-2 from samples and CFL-2-HRP conjugate compete for the anti-CFL-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CFL-2 from the sample, fewer sites are left to bind CFL-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFL-2 concentration in each sample is interpolated from this standard curve.

33946278

NP_619579.1

NM_138638.4

Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Caspase Cascade In Apoptosis Pathway (137974); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590); G13 Signaling Pathway (198800); Leptin Signaling Pathway (672456); Pertussis Pathway (218111); Pertussis Pathway (218099); Regulation Of Actin Cytoskeleton Pathway (198874)

This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]

Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. Protein type: Nuclear receptor co-regulator; Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 14q12. Cellular Component: I band; extracellular space; nuclear matrix; actin cytoskeleton. Molecular Function: protein binding; actin binding. Biological Process: muscle maintenance; actin filament depolymerization; positive regulation of actin filament depolymerization; sarcomere organization. Disease: Nemaline Myopathy 7

48-Strip-Wells

470 USD

96-Strip-Wells

675