Guinea pig Interleukin 17A ELISA Kit | MyBioSource MBS727428 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig Interleukin 17A ELISA Kit

catalog :

MBS727428

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS727428

products type :

ELISA Kit

products full name :

Guinea pig Interleukin 17A ELISA Kit

products short name :

Interleukin 17A

other names :

interleukin-17A; Interleukin-17A; interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8

products gene name :

IL-17A

other gene names :

IL17A; IL17A; IL17; CTLA8; IL-17; IL-17A; CTLA8; IL17; IL-17; IL-17A; CTLA-8

uniprot entry name :

IL17_HUMAN

reactivity :

Guinea Pig

specificity :

This assay has high sensitivity and excellent specificity for detection of IL-17A. No significant cross-reactivity or interference between IL-17A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-17A and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This IL-17A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig IL-17A. This ELISA kit for research use only, not for therapeutic or diagnostic applications

products categories :

Immunology

products description :

Principle of the assay: IL-17A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-17A. Standards or samples are then added to the microtiter plate wells and IL-17A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-17A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-17A are added to each well to "sandwich" the IL-17A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-17A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-17A concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4504651

ncbi acc num :

NP_002181.1

ncbi gb acc num :

NM_002190.2

ncbi mol weight :

17,504 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL17 Signaling Pathway (920968); IL23-mediated Signaling Events Pathway (138000); IL27-mediated Signaling Events Pathway (137929); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269); TCR Signaling Pathway (198862)

ncbi summary :

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

uniprot summary :

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p12. Cellular Component: extracellular space; external side of plasma membrane. Molecular Function: cytokine activity. Biological Process: cell death; cell-cell signaling; positive regulation of osteoclast differentiation; apoptosis; positive regulation of interleukin-23 production; protein amino acid glycosylation; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage