ELISA/assay

Mouse Anti-Histone Antibody ELISA Kit

MBS727399

48-Strip-Wells

470 USD

ELISA Kit

Mouse Anti-Histone Antibody ELISA Kit

Anti-Histone Antibody

histone; Histone H2A.Z; histone H2A.Z; H2AZ histone; H2A histone family, member Z

AHA

H2AFZ; H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1; H2AZ; H2A/z

H2AZ_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of HISAb. No significant cross-reactivity or interference between HISAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HISAb and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ug/ml.

Signal Transduction

Intended Uses: This HISAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HISAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay HISAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Histone antigen and an HISAb-HRP conjugate. The assay sample and buffer are incubated together with HISAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HISAb concentration since HISAb from samples and HISAb-HRP conjugate compete for the Histone antigen binding site. Since the number of sites is limited, as more sites are occupied by HISAb from the sample, fewer sites are left to bind HISAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HISAb concentration in each sample is interpolated from this standard curve.

3649600

AAC61625.1

13,553 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (366238); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromatin Modifying Enzymes Pathway (941403); Chromatin Organization Pathway (941402)

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: Barr body; nucleosome; nuclear heterochromatin; nucleus. Molecular Function: protein binding; nucleosomal DNA binding; chromatin DNA binding; protein heterodimerization activity. Biological Process: positive regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

470 USD

96-Strip-Wells

675