Mouse Lactoferrin ELISA Kit | MyBioSource MBS727334 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Lactoferrin ELISA Kit

catalog :

MBS727334

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS727334

products type :

ELISA Kit

products full name :

Mouse Lactoferrin ELISA Kit

products short name :

Lactoferrin

products name syn :

Mouse Lactoferrin ELISA Kit; Lactoferrin; Lactoferrin (Mouse)

other names :

lactotransferrin isoform 2; Lactotransferrin; lactotransferrin; talalactoferrin; OTTHUMP00000209779; OTTHUMP00000209780; OTTHUMP00000209781; neutrophil lactoferrin; growth-inhibiting protein 12; lactotransferrin; Talalactoferrin

products gene name :

LTF/LF

other gene names :

LTF; LTF; LF; HLF2; GIG12; LF

uniprot entry name :

TRFL_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of LTF. No significant cross-reactivity or interference between LTF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LTF and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

products categories :

Mouse ELISA Kit

products description :

Intended Uses This LTF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LTF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: LTF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LTF antibody and an LTF-HRP conjugate. The assay sample and buffer are incubated together with LTF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTF concentration since LTF from samples and LTF-HRP conjugate compete for the anti-LTF antibody binding site. Since the number of sites is limited, as more sites are occupied by LTF from the sample, fewer sites are left to bind LTF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTF concentration in each sample is interpolated from this standard curve.

ncbi gi num :

312433998

ncbi acc num :

NP_001186078.1

ncbi gb acc num :

NM_001199149.1

uniprot acc num :

P02788

ncbi mol weight :

78,182 Da

ncbi pathways :

Amyloids Pathway (366238)

ncbi summary :

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

uniprot summary :

Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Ref.21Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Ref.21Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Ref.21The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Ref.21. Catalytic activity: Preferential at -Arg-Ser-Arg-Arg- - and -Arg-Arg-Ser-Arg- -, and of Z-Phe-Arg- -aminomethylcoumarin. Subunit structure: Monomer. Subcellular location: Secreted. Sequence similarities: Belongs to the transferrin family.Contains 2 transferrin-like domains.

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage