catalog number :
MBS727326
products type :
ELISA Kit
products full name :
Mouse Glucose Transporter 4 ELISA Kit
products short name :
[Glucose Transporter 4]
other names :
[glut4, partial; Glut4; Glut4]
products gene name :
[GLUT4]
uniprot entry name :
Q58HK0_SHEEP
specificity :
This assay has high sensitivity and excellent specificity for detection of GLUT3. No significant cross-reactivity or interference between GLUT3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLUT3 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Signal Transduction
products description :
Intended Uses: This GLUT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GLUT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GLUT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GLUT3 antibody and an GLUT3-HRP conjugate. The assay sample and buffer are incubated together with GLUT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLUT3 concentration since GLUT3 from samples and GLUT3-HRP conjugate compete for the anti-GLUT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLUT3 from the sample, fewer sites are left to bind GLUT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT3 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAX51300.1
ncbi mol weight :
20,643 Da
size4 :
10x96-Strip-Wells