ELISA/assay

Human Plasminogen Activator Inhibitor 2 ELISA Kit

MBS727325

48-Strip-Wells

470 USD

ELISA Kit

Human Plasminogen Activator Inhibitor 2 ELISA Kit

Plasminogen Activator Inhibitor 2

plasminogen activator inhibitor 2; Plasminogen activator inhibitor 2; plasminogen activator inhibitor 2; serpin B2; monocyte Arg-serpin; urokinase inhibitor; placental plasminogen activator inhibitor; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Monocyte Arg-serpin; Placental plasminogen activator inhibitor; Serpin B2; Urokinase inhibitor

PAI2

SERPINB2; SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201; PAI2; PLANH2; PAI-2

PAI2_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of PAI-2. No significant cross-reactivity or interference between PAI-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PAI-2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Signal Transduction

Intended Uses: This PAI-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PAI-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay PAI-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAI-2 antibody and an PAI-2-HRP conjugate. The assay sample and buffer are incubated together with PAI-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAI-2 concentration since PAI-2 from samples and PAI-2-HRP conjugate compete for the anti-PAI-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAI-2 from the sample, fewer sites are left to bind PAI-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAI-2 concentration in each sample is interpolated from this standard curve.

219689110

NP_001137290.1

NM_001143818.1

46,596 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Blood Clotting Cascade Pathway (198840); Dissolution Of Fibrin Clot Pathway (106061); Hemostasis Pathway (106028); Senescence And Autophagy Pathway (198780)

SERPINB2: Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell- derived PAI-1. Belongs to the serpin family. Ov-serpin subfamily. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 18q21.3. Cellular Component: extracellular space; cytoplasm; extracellular region; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: fibrinolysis; blood coagulation; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675