catalog number :
MBS727250
products type :
ELISA Kit
products full name :
Guinea pig Terminal complement complex C5b-9 ELISA Kit
products short name :
Terminal complement complex C5b-9
products gene name :
TCC C5b-9
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This C5b9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig C5b9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: C5b9 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C5b9. Standards or samples are then added to the microtiter plate wells and C5b9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C5b9 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for C5b9 are added to each well to "sandwich" the C5b9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C5b9 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C5b9 concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)