ELISA/assay

Mouse Follicle Stimulating Hormone ELISA Kit

MBS727159

48-Strip-Wells

470 USD

ELISA Kit

Mouse Follicle Stimulating Hormone ELISA Kit

Follicle Stimulating Hormone

FSH; Bromodomain-containing protein 2; O27.1.1; Really interesting new gene 3 protein

FSH

BRD2; KIAA9001; RING3

BRD2_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of FSH. No significant cross-reactivity or interference between FSH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FSH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses This FSH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FSH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: FSH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FSH. Standards or samples are then added to the microtiter plate wells and FSH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FSH are added to each well to "sandwich” the FSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FSH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSH concentration in each sample is interpolated from this standard curve.

2980663

CAA43996.1

P25440

74,881 Da

BRD2: an atypical protein kinase with two bromodomains that bind to acetylated lysine residues. Specifically interacts with transcriptionally active chromatin. Elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. Interacts with E2F1 and with histone H4 acetylated at Lys-13. May play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses. Two isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; Kinase, protein; ATYPICAL group; BRD family. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; chromatin binding. Biological Process: regulation of transcription from RNA polymerase II promoter; nucleosome assembly; transcription, DNA-dependent; spermatogenesis; chromatin modification

48-Strip-Wells

470 USD

96-Strip-Wells

675