ELISA/assay

Rat Orexins ELISA Kit

MBS727107

48-Strip-Wells

470 USD

ELISA Kit

Rat Orexins ELISA Kit

Orexins

orexin; Orexin; orexin; prepro-orexin; hypocretin (orexin) neuropeptide precursor; Hypocretin; Hcrt

Orexins

HCRT; HCRT; OX; PPOX; NRCLP1; OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2

OREX_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This Orexins ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Orexins. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the Assay: Orexins ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Orexins antibody and an Orexins-HRP conjugate. The assay sample and buffer are incubated together with Orexins-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Orexins concentration since Orexins from samples and Orexins-HRP conjugate compete for the anti-Orexins antibody binding site. Since the number of sites is limited, as more sites are occupied by Orexins from the sample, fewer sites are left to bind Orexins-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Orexins concentration in each sample is interpolated from this standard curve.

4557635

NP_001515.1

NM_001524.1

13,363 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway (106362); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]

HCRT: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1). Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Belongs to the orexin family. Protein type: Hormone. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: synaptic vesicle; rough endoplasmic reticulum; perinuclear region of cytoplasm; extracellular region; cell junction; secretory granule. Molecular Function: type 2 hypocretin receptor binding; type 1 hypocretin receptor binding. Biological Process: synaptic transmission; elevation of cytosolic calcium ion concentration; negative regulation of transmission of nerve impulse; neuropeptide signaling pathway; eating behavior; negative regulation of DNA replication; negative regulation of potassium ion transport; protein kinase C activation; regulation of neurotransmitter secretion; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of calcium ion transport; regulation of excitatory postsynaptic membrane potential; positive regulation of transmission of nerve impulse. Disease: Narcolepsy 1

48-Strip-Wells

470 USD

96-Strip-Wells

675