catalog number :
MBS727076
products type :
ELISA Kit
products full name :
Mouse Inhibin ELISA Kit
products short name :
Inhibin
other names :
Inh; Inhibitor of prohead protease gp21; Inh; inhibitor of prohead protease gp21; Inhibitor of prohead protease gp21
other gene names :
inh; inh
uniprot entry name :
Q6U9C9_9CAUD
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive
other info2 :
Intended Uses: This INH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse INH. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This INH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse INH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Neurobiology
products description :
Principle of the Assay: INH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-INH antibody and an INH-HRP conjugate. The assay sample and buffer are incubated together with INH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INH concentration since INH from samples and INH-HRP conjugate compete for the anti-INH antibody binding site. Since the number of sites is limited, as more sites are occupied by INH from the sample, fewer sites are left to bind INH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INH concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_932528.1
ncbi gb acc num :
NC_005135.1
ncbi mol weight :
27,919 Da