ELISA/assay

Human Heat Shock Protein 27 ELISA Kit

MBS727021

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Heat Shock Protein 27 ELISA Kit

Heat Shock Protein 27

HSP27; Heat shock protein beta-1; heat shock protein beta-1; HSP 27; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein; epididymis secretory protein Li 102; heat shock 27kDa protein 1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

HSP27

HSPB1; HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; HSP27; HSP28; HspB1; HSP 27; SRP27

HSPB1_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Highest Standards: 50ng/mL. Concentration of Standards: Concentration of Standards: 0,2.5,5.0,10,25,50ng/mL

Signal Transduction

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This HSP-27 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HSP-27. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY HSP-27 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HSP-27. Standards or samples are then added to the microtiter plate wells and HSP-27 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HSP-27 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HSP-27 are added to each well to “sandwich” the HSP-27 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HSP-27 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-27 concentration in each sample is interpolated from this standard curve

11036357

BAB17232.1

22,783 Da

AUF1 (hnRNP D0) Destabilizes MRNA Pathway (187212); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); FAS Pathway And Stress Induction Of HSP Regulation (198894); Gene Expression Pathway (105937); IL-3 Signaling Pathway (198881); IL-6 Signaling Pathway (198864); MAPK Signaling Pathway (198779)

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock. Protein type: Motility/polarity/chemotaxis; Heat shock protein; Chaperone. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; Z disc; cytosol; nucleus. Molecular Function: protein kinase C inhibitor activity; identical protein binding; ubiquitin binding; protein binding; protein kinase C binding; protein kinase binding. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; response to virus; positive regulation of blood vessel endothelial cell migration; response to unfolded protein; positive regulation of interleukin-1 beta production; retinal homeostasis; positive regulation of angiogenesis; negative regulation of protein kinase activity; gene expression; regulation of I-kappaB kinase/NF-kappaB cascade; vascular endothelial growth factor receptor signaling pathway; cell motility; regulation of translational initiation; negative regulation of apoptosis. Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675