ELISA/assay

Human Transforming Growth Factor beta 3 ELISA Kit

MBS726974

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Transforming Growth Factor beta 3 ELISA Kit

Transforming Growth Factor beta 3

Human Transforming Growth Factor b 3 ELISA Kit

TPA: transforming growth factor beta-3; transforming growth factor beta-3; transforming growth factor, beta 3

TGFb3

TGFb3

TGFB3

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

Intended Uses: This TGFbeta3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TGFbeta3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the Assay: TGFbeta3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGFbeta3 antibody and an TGFbeta3-HRP conjugate. The assay sample and buffer are incubated together with TGFbeta3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGFbeta3 concentration since TGFbeta3 from samples and TGFbeta3-HRP conjugate compete for the anti-TGFbeta3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TGFbeta3 from the sample, fewer sites are left to bind TGFbeta3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFbeta3 concentration in each sample is interpolated from this standard curve.

296482901

DAA25016.1

Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Cell Cycle Pathway (84293); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (84351); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (84341); Colorectal Cancer Pathway (518)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675