catalog number :
MBS726968
products type :
ELISA Kit
products full name :
Mouse Coagulation factor Xa ELISA Kit
products short name :
Coagulation factor Xa
other names :
Chain L, Human Coagulation Factor Xa In Complex With M55192
specificity :
This assay has high sensitivity and excellent specificity for detection of FXa. No significant cross-reactivity or interference between FXa and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FXa and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This FXa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FXa. This ELISA kit for research use only, not for therapeutic or diagnostic applications
products description :
Principle of the assay: FXa ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FXa antibody and an FXa-HRP conjugate. The assay sample and buffer are incubated together with FXa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FXa concentration since FXa from samples and FXa-HRP conjugate compete for the anti-FXa antibody binding site. Since the number of sites is limited, as more sites are occupied by FXa from the sample, fewer sites are left to bind FXa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FXa concentration in each sample is interpolated from this standard curve.