Rat Growth Hormone 2 ELISA Kit | MyBioSource MBS726967 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Growth Hormone 2 ELISA Kit

catalog :

MBS726967

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS726967

products type :

ELISA Kit

products full name :

Rat Growth Hormone 2 ELISA Kit

products short name :

Growth Hormone 2

other names :

Growth hormone 2; Growth hormone variant; growth hormone variant; placenta-specific growth hormone; placental-specific growth hormone; growth hormone 2; Growth hormone 2; Placenta-specific growth hormone

products gene name :

GH2

other gene names :

GH2; GH2; GHL; GHV; GH-V; hGH-V; GH-V

uniprot entry name :

SOM2_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of GH2. No significant cross-reactivity or interference between GH2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GH2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This GH2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GH2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

. Principle of the assay: GH2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GH2 antibody and an GH2-HRP conjugate. The assay sample and buffer are incubated together with GH2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GH2 concentration since GH2 from samples and GH2-HRP conjugate compete for the anti-GH2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GH2 from the sample, fewer sites are left to bind GH2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GH2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

18088461

ncbi acc num :

AAH20760.1

ncbi mol weight :

27,101 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Signaling By ERBB4 Pathway (530744)

ncbi summary :

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. As in the case of its pituitary counterpart, growth hormone 1, the predominant isoform of this particular family member shows similar somatogenic activity, with reduced lactogenic activity. Mutations in this gene lead to placental growth hormone/lactogen deficiency. [provided by RefSeq, Jul 2008]

uniprot summary :

GH2: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q24.2. Cellular Component: extracellular region. Molecular Function: hormone activity

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage