Rat Haptoglobin precursor ELISA Kit | MyBioSource MBS726952 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Haptoglobin precursor ELISA Kit

catalog :

MBS726952

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS726952

products type :

ELISA Kit

products full name :

Rat Haptoglobin precursor ELISA Kit

products short name :

Haptoglobin precursor

other names :

haptoglobin; Haptoglobin; haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide; haptoglobin; ZonulinCleaved into the following 2 chains:Haptoglobin alpha chain; Haptoglobin beta chain

products gene name :

Pre-Hpt

other gene names :

HP; HP; BP; HPA1S; HP2ALPHA2

uniprot entry name :

HPT_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of HP. No significant cross-reactivity or interference between HP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HP and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This HP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: HP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HP antibody and an HP-HRP conjugate. The assay sample and buffer are incubated together with HP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HP concentration since HP from samples and HP-HRP conjugate compete for the anti-HP antibody binding site. Since the number of sites is limited, as more sites are occupied by HP from the sample, fewer sites are left to bind HP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HP concentration in each sample is interpolated from this standard curve.

ncbi gi num :

386783

ncbi acc num :

AAA88080.1

ncbi mol weight :

38,452 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16q22.2. Cellular Component: extracellular space; extracellular region. Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity. Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; immune system process; metabolic process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response. Disease: Anhaptoglobinemia

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage