catalog number :
MBS726928
products type :
ELISA Kit
products full name :
Mouse Total Immunoglobulin ELISA Kit
products short name :
Total Immunoglobulin
other names :
tig; Trigger factor; PPIase
other gene names :
tig; IPF_798; TF
uniprot entry name :
A8YMD6_MICAE
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 mg/ml.
products categories :
Immunology
products description :
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This TIG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TIG. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY TIG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TIG antibody and an TIG-HRP conjugate. The assay sample and buffer are incubated together with TIG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TIG concentration since TIG from samples and TIG-HRP conjugate compete for the anti-TIG antibody binding site. Since the number of sites is limited, as more sites are occupied by TIG from the sample, fewer sites are left to bind TIG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIG concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAO91361.1
ncbi mol weight :
51,856 Da