ELISA/assay

Mouse Complement Component 1 Q Receptor ELISA Kit

MBS726892

48-Strip-Wells

470 USD

ELISA Kit

Mouse Complement Component 1 Q Receptor ELISA Kit

Complement Component 1 Q Receptor

complement component C1q receptor; Complement component C1q receptor; complement component C1q receptor; C1qR; CD93 antigen; C1q receptor 1; C1q/MBL/SPA receptor; matrix-remodelling associated 4; matrix-remodeling-associated protein 4; complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; CD93 molecule; C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; Complement component 1 q subcomponent receptor 1; Matrix-remodeling-associated protein 4; CD_antigen: CD93

C1qR1

CD93; CD93; C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1; C1QR1; MXRA4; C1qR; C1qR(p); C1qRp

C1QR1_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This C1QR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C1QR. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY C1QR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C1QR antibody and an C1QR-HRP conjugate. The assay sample and buffer are incubated together with C1QR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C1QR concentration since C1QR from samples and C1QR-HRP conjugate compete for the anti-C1QR antibody binding site. Since the number of sites is limited, as more sites are occupied by C1QR from the sample, fewer sites are left to bind C1QR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1QR concentration in each sample is interpolated from this standard curve.

88758613

NP_036204.2

NM_012072.3

68,560 Da

The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. [provided by RefSeq, Jul 2008]

CD93: Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 20p11.21. Cellular Component: cell surface; cytoplasmic membrane-bound vesicle; plasma membrane; integral to membrane. Molecular Function: protein binding; complement component C1q binding; receptor activity; calcium ion binding; carbohydrate binding. Biological Process: macrophage activation; cell-cell adhesion; viral reproduction; phagocytosis

48-Strip-Wells

470 USD

96-Strip-Wells

675