ELISA/assay

Human Defensin Alpha 5, Paneth Cell Specific ELISA Kit

MBS726865

48-Strip-Wells

470 USD

ELISA Kit

Human Defensin Alpha 5, Paneth Cell Specific ELISA Kit

Defensin Alpha 5, Paneth Cell Specific

defensin, alpha 5, Paneth cell-specific; Defensin-5; defensin-5; HD5(20-94); defensin 5; defensin, alpha 5, preproprotein; defensin, alpha 5, Paneth cell-specific; Defensin, alpha 5; HD5(20-94)Cleaved into the following 4 chains:HD5(23-94); HD5(29-94); HD5(56-94); HD5(63-94)

DEFAlpha 5

DEFA5; DEFA5; DEF5; HD-5; DEF5

DEF5_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Immunology

Intended Uses: This DEFa5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DEFa5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: DEFa5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DEFa5. Standards or samples are then added to the microtiter plate wells and DEFa5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DEFa5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DEFa5 are added to each well to "sandwich" the DEFa5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DEFa5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFa5 concentration in each sample is interpolated from this standard curve.

50057850

AAT68886.1

10,071 Da

Alpha-defensins Pathway (530758); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Jul 2008]

DEFA5: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. Belongs to the alpha-defensin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; transport vesicle; Golgi lumen; extracellular region. Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus

48-Strip-Wells

470 USD

96-Strip-Wells

675