catalog number :
MBS726722
products type :
ELISA Kit
products full name :
Guinea pig Kidney Injury Molecule 1 ELISA Kit
products short name :
Kidney Injury Molecule 1
other names :
kidney injury molecule 1; Kidney injury molecule 1; Kidney injury molecule 1; Uncharacterized protein; Zgc:92647
products gene name :
KIM1
other gene names :
havcr1; kim1; zgc:92647
uniprot entry name :
Q6DH50_DANRE
specificity :
This assay has high sensitivity and excellent specificity for detection of KIM-1. No significant cross-reactivity or interference between KIM-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KIM-1 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Sensitivity: 1.0 pg/mL.
products categories :
Immunology
products description :
Intended Uses This KIM-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea KIM-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: KIM-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KIM-1 antibody and an KIM-1-HRP conjugate. The assay sample and buffer are incubated together with KIM-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KIM-1 concentration since KIM-1 from samples and KIM-1-HRP conjugate compete for the anti-KIM-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KIM-1 from the sample, fewer sites are left to bind KIM-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KIM-1 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ABP97434.1
ncbi mol weight :
29,575 Da
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)