ELISA/assay

Rat Adenylyl Cyclase Associated Protein 1 ELISA Kit

MBS726685

48-Strip-Wells

470 USD

ELISA Kit

Rat Adenylyl Cyclase Associated Protein 1 ELISA Kit

Adenylyl Cyclase Associated Protein 1

CAP1; Protein DJ-1; protein DJ-1; fertility protein SP22; contraception-associated protein 1; parkinson disease protein 7 homolog; Parkinson disease (autosomal recessive, early onset) 7; parkinson protein 7; Contraception-associated protein 1; Protein CAP1; Fertility protein SP22; Parkinson disease protein 7 homolog

CAP1

Park7; Park7; Dj1; CAP1; DJ-1; SP22; Cap1; Protein CAP1

PARK7_RAT

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive

Intended Uses: This ACAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ACAP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This ACAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ACAP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: ACAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACAP1 antibody and an ACAP1-HRP conjugate. The assay sample and buffer are incubated together with ACAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACAP1 concentration since ACAP1 from samples and ACAP1-HRP conjugate compete for the anti-ACAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACAP1 from the sample, fewer sites are left to bind ACAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACAP1 concentration in each sample is interpolated from this standard curve.

3250916

CAA07434.1

O88767

19,974 Da

Parkinson's Disease Pathway (83490)

displays a specific localization pattern to the anterior-ventral surface of the equatorial segment; may play a role in sperm-egg interactions and fertilization [RGD, Feb 2006]

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration. Protein type: EC 3.4.-.-; Nuclear receptor co-regulator; Tumor suppressor; Transcription regulation. Cellular Component: PML body; mitochondrion; axon; endoplasmic reticulum; cytoplasm; plasma membrane; chromatin; cytosol; nucleus; mitochondrial respiratory chain complex I; lipid raft. Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; mercury ion binding; transcription coactivator activity; transcription factor binding; peptidase activity; mRNA binding; protein binding; copper ion binding; enzyme binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding. Biological Process: synaptic transmission, dopaminergic; negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; regulation of mitochondrial membrane potential; single fertilization; regulation of neuron apoptosis; dopamine uptake; negative regulation of neuron apoptosis; inflammatory response; negative regulation of protein binding; response to drug; mitochondrion organization and biogenesis; protein stabilization; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of peptidyl-serine phosphorylation; negative regulation of protein sumoylation; methylglyoxal catabolic process to D-lactate; membrane depolarization; fertilization; response to hydrogen peroxide; detoxification of mercury ion; regulation of inflammatory response; negative regulation of ubiquitin-protein ligase activity; hydrogen peroxide metabolic process; autophagy; lactate biosynthetic process; negative regulation of protein kinase activity; positive regulation of transcription factor activity; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

675