catalog number :
MBS726626
products type :
ELISA Kit
products full name :
Mouse Thyroid stimulating hormone TSH ELSA ELISA Kit
products short name :
Thyroid stimulating hormone TSH ELSA
other names :
Tsh; Temperature-sensitive hemagglutinin tsh autotransporter; Autotransporter tshCleaved into the following 2 chains:Temperature-sensitive hemagglutinin tsh; Temperature-sensitive hemagglutinin tsh translocator
uniprot entry name :
TSH_ECOLX
specificity :
This assay has high sensitivity and excellent specificity for detection of TSH. No significant cross-reactivity or interference between TSH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TSH and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This TSH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TSH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: TSH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TSH antibody and an TSH-HRP conjugate. The assay sample and buffer are incubated together with TSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TSH concentration since TSH from samples and TSH-HRP conjugate compete for the anti-TSH antibody binding site. Since the number of sites is limited, as more sites are occupied by TSH from the sample, fewer sites are left to bind TSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSH concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAA24698.1
ncbi mol weight :
148,227 Da