ELISA/assay

Rabbit Interleukin 12 ELISA Kit

MBS726583

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Interleukin 12 ELISA Kit

Interleukin 12

Rabbit Interleukin 12 ELISA Kit; Interleukin 12; Interleukin 12 (Rabbit)

interleukin 12; Interleukin-12 subunit alpha; interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; natural killer cell stimulatory factor 1, 35 kD subunit; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1

IL-12/P70

IL12A; IL12A; P35; CLMF; NFSK; NKSF1; IL-12A; NKSF1; IL-12A; CLMF p35; NKSF1

IL12A_HUMAN

Rabbit

Store all reagents at 2-8 degree C

Assay Type: Sandwich

Rabbit ELISA Kit

Intended Uses: This IL-12 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IL-12in the sample, this IL-12ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-12concentration. The concentration of IL-12in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This IL-12enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-12. Standards or samples are then added to the microtiter plate wells and IL-12if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-12present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-12are added to each well to "sandwich" the IL-12immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain IL-12and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

4323579

AAD16432.1

P29459

24,874 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Subunit structure: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12. Ref.8. Subcellular location: Secreted. Induction: Down-regulated in response to enterovirus 71 (EV71) infection. Ref.7. Sequence similarities: Belongs to the IL-6 superfamily. Sequence caution: The sequence AAA59937.1 differs from that shown. Reason: Erroneous initiation.

48-Strip-Wells

470 USD

96-Strip-Wells

675