ELISA/assay

Rat Cluster of Differentiation 200 ELISA Kit

MBS726520

48-Strip-Wells

470 USD

ELISA Kit

Rat Cluster of Differentiation 200 ELISA Kit

Cluster of Differentiation 200

chemokine; C-C motif chemokine 15; C-C motif chemokine 15; leukotactin 1; chemokine CC-2; new CC chemokine 3; small-inducible cytokine A15; macrophage inflammatory protein 5; small inducible cytokine subfamily A (Cys-Cys), member 15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; Leukotactin-1; LKN-1; MIP-1 delta; Macrophage inflammatory protein 5; MIP-5; Mrp-2b; NCC-3; Small-inducible cytokine A15Cleaved into the following 3 chains:CCL15(22-92); CCL15(25-92); CCL15(29-92)

CD200

CCL15; CCL15; LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta; MIP5; NCC3; SCYA15; HCC-2; LKN-1; MIP-5

CCL15_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of CD200. No significant cross-reactivity or interference between CD200 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD200 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CD200 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CD200. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: CD200 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD200 antibody and an CD200-HRP conjugate. The assay sample and buffer are incubated together with CD200-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD200 concentration since CD200 from samples and CD200-HRP conjugate compete for the anti-CD200 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD200 from the sample, fewer sites are left to bind CD200-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD200 concentration in each sample is interpolated from this standard curve.

4204910

AAD10847.1

12,248 Da

Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460)

This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013]

CCL15: Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. Belongs to the intercrine beta (chemokine CC) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space. Molecular Function: heparin binding; chemokine activity; receptor binding; chemoattractant activity. Biological Process: cellular calcium ion homeostasis; cell-cell signaling; positive chemotaxis; immune response; signal transduction; chemotaxis

48-Strip-Wells

470 USD

96-Strip-Wells

675