ELISA/assay

Mouse Inhibin alpha ELISA Kit

MBS726518

48-Strip-Wells

470 USD

ELISA Kit

Mouse Inhibin alpha ELISA Kit

Inhibin alpha

Mouse Inhibin alpha ELISA Kit; Inhibin alpha; Inhibin a ELISA Kit; Inhibin ELISA Kit; Inhibin alpha ELISA Kit (Mouse)

inhibin alpha

INB-alpha

INB-a; INB-

Mouse

This assay has high sensitivity and excellent specificity for detection of INHalpha. No significant cross-reactivity or interference between INHalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between INHalpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This INHalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse INHalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Mouse ELISA Kit

Principle of the assay: INHalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for INHalpha. Standards or samples are then added to the microtiter plate wells and INHalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of INHalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for INHalpha are added to each well to "sandwich" the INHalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain INHalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INHalpha concentration in each sample is interpolated from this standard curve.

208436014

ACI28454

This gene encodes the alpha subunit of inhibins A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.[provided by RefSeq, Dec 2010]

INHA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2q35. Cellular Component: photoreceptor outer segment; photoreceptor inner segment; cell soma; extracellular region. Molecular Function: protein binding; growth factor activity; protein heterodimerization activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding. Biological Process: negative regulation of B cell differentiation; hemoglobin biosynthetic process; nervous system development; regulation of cell cycle; male gonad development; signal transduction; negative regulation of interferon-gamma biosynthetic process; regulation of cell proliferation; negative regulation of follicle-stimulating hormone secretion; negative regulation of cell cycle; regulation of apoptosis; response to external stimulus; cell surface receptor linked signal transduction; cell-cell signaling; ovarian follicle development; negative regulation of macrophage differentiation; regulation of MAPKKK cascade; negative regulation of phosphorylation; erythrocyte differentiation; positive regulation of follicle-stimulating hormone secretion; cell cycle arrest; cell differentiation; cell development; skeletal development

48-Strip-Wells

470 USD

96-Strip-Wells

675