Human Cofilin 1 (Non Muscle) ELISA Kit | MyBioSource MBS726481 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cofilin 1 (Non Muscle) ELISA Kit

catalog :

MBS726481

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS726481

products type :

ELISA Kit

products full name :

Human Cofilin 1 (Non Muscle) ELISA Kit

products short name :

Cofilin 1 (Non Muscle)

other names :

cofilin-1; Cofilin-1; cofilin-1; p18; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15; cofilin 1 (non-muscle); 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform

products gene name :

CFL1

other gene names :

CFL1; CFL1; CFL; HEL-S-15; CFL; p18

uniprot entry name :

COF1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of CFL-1. No significant cross-reactivity or interference between CFL-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CFL-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

products description :

Intended Uses: This CFL-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CFL-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CFL-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CFL-1. Standards or samples are then added to the microtiter plate wells and CFL-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CFL-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CFL-1 are added to each well to "sandwich" the CFL-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CFL-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CFL-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5031635

ncbi acc num :

NP_005498.1

ncbi gb acc num :

NM_005507.2

ncbi mol weight :

18,502 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); BDNF Signaling Pathway (712093); CDC42 Signaling Events Pathway (137994); CXCR4-mediated Signaling Events Pathway (137910); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1016174); EPHB-mediated Forward Signaling Pathway (1016176); Fc Gamma R-mediated Phagocytosis Pathway (114228)

ncbi summary :

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

uniprot summary :

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. Protein type: Cytoskeletal; Nuclear receptor co-regulator; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; cortical actin cytoskeleton; focal adhesion; nuclear matrix; membrane; cytoplasm; intercellular junction; nucleus; vesicle. Molecular Function: protein binding; actin binding. Biological Process: actin filament depolymerization; axon guidance; platelet activation; positive regulation of actin filament depolymerization; response to virus; cytoskeleton organization and biogenesis; negative regulation of cell size; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; protein import into nucleus; establishment of cell polarity; ephrin receptor signaling pathway; regulation of cell morphogenesis; innate immune response; actin cytoskeleton organization and biogenesis; blood coagulation; neural crest cell migration; negative regulation of apoptosis

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage