ELISA/assay

Human 25-Dihydroxy vitamin D3 ELISA Kit

MBS726414

48-Strip-Wells

470 USD

ELISA Kit

Human 25-Dihydroxy vitamin D3 ELISA Kit

25-Dihydroxy vitamin D3

Human 25-Dihydroxy vitamin D3 ELISA Kit; 25-Dihydroxy vitamin D3; 25-Dihydroxy vitamin D3 (Human)

25 HVD3

Human

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This 25OHVD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 25OHVD3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Human ELISA Kit

Principle of the Assay: 25OHVD3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-25OHVD3 antibody and an 25OHVD3-HRP conjugate. The assay sample and buffer are incubated together with 25OHVD3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 25OHVD3 concentration since 25OHVD3 from samples and 25OHVD3-HRP conjugate compete for the anti-25OHVD3 antibody binding site. Since the number of sites is limited, as more sites are occupied by 25OHVD3 from the sample, fewer sites are left to bind 25OHVD3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 25OHVD3 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675