ELISA/assay

Human Activating Transcription Factor 4 ELISA Kit

MBS726369

96 Strip Wells

675 USD

ELISA Kit

Human Activating Transcription Factor 4 ELISA Kit

Activating Transcription Factor 4

ATF4; ATF4 protein; cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element-binding protein 67; activating transcription factor 4; ATF4 protein; Activating transcription factor 4 (Tax-responsive enhancer element B67); Activating transcription factor 4 (Tax-responsive enhancer element B67), isoform CRA_a

ATF4

ATF4; ATF4; CREB2; TXREB; CREB-2; TAXREB67; hCG_41721

Q96AQ3_HUMAN

Human

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Epigenetics and Nuclear Signaling

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. INTENDED USE This ATF4/CREB2/TXREB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATF4/CREB2/TXREB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY ATF4/CREB2/TXREB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF4/CREB2/TXREB antibody and an ATF4/CREB2/TXREB-HRP conjugate. The assay sample and buffer are incubated together with ATF4/CREB2/TXREB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF4/CREB2/TXREB concentration since ATF4/CREB2/TXREB from samples and ATF4/CREB2/TXREB-HRP conjugate compete for the anti-ATF4/CREB2/TXREB antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF4/CREB2/TXREB from the sample, fewer sites are left to bind ATF4/CREB2/TXREB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF4/CREB2/TXREB concentration in each sample is interpolated from this standard curve.

47678299

CAG30270.1

Q96AQ3

38,590 Da

ATF4 Activates Genes Pathway 530772!!ATF6-alpha Activates Chaperone Genes Pathway 530770!!ATF6-alpha Activates Chaperones Pathway 105905!!Adrenergic Signaling In Cardiomyocytes Pathway 908257!!Adrenergic Signaling In Cardiomyocytes Pathway 909696!!Alcoholism Pathway 585563!!Alcoholism Pathway 587116!!Amphetamine Addiction Pathway 547607!!Amphetamine Addiction Pathway 550546!!Cholinergic Synapse Pathway 217716

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

96 Strip Wells

675 USD