ELISA/assay

Mouse Arrestin Beta 2 ELISA Kit

MBS726347

48-Strip-Wells

470 USD

ELISA Kit

Mouse Arrestin Beta 2 ELISA Kit

Arrestin Beta 2

arrestin, beta 2; arrestin, beta 2; arrestin 2; beta-Arrestin 2; arrestin, beta 2

ARRbeta2

arrb2; arb2; arr2; barr2; arrestin; betaarr2

Mouse

This assay has high sensitivity and excellent specificity for detection of ARRbeta2. No significant cross-reactivity or interference between ARRbeta2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARRbeta2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This ARRbeta2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ARRbeta2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

. Principle of the assay: ARRbeta2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARRbeta2 antibody and an ARRbeta2-HRP conjugate. The assay sample and buffer are incubated together with ARRbeta2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARRbeta2 concentration since ARRbeta2 from samples and ARRbeta2-HRP conjugate compete for the anti-ARRbeta2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARRbeta2 from the sample, fewer sites are left to bind ARRbeta2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARRbeta2 concentration in each sample is interpolated from this standard curve.

154147682

NP_001093721.1

NM_001100251.1

Endocytosis Pathway (102297); Endocytosis Pathway (102181); MAPK Signaling Pathway (84769); MAPK Signaling Pathway (456); Phototransduction Pathway (149832); Phototransduction Pathway (148660)

48-Strip-Wells

470 USD

96-Strip-Wells

675