ELISA/assay

Rat Ferritin Heavy Polypeptide ELISA Kit

MBS726243

48-Strip-Wells

470 USD

ELISA Kit

Rat Ferritin Heavy Polypeptide ELISA Kit

Ferritin Heavy Polypeptide

ferritin, heavy polypeptide 1, isoform CRA_a; Ferritin heavy chain; ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein; ferritin, heavy polypeptide 1; Cell proliferation-inducing gene 15 proteinCleaved into the following chain:Ferritin heavy chain, N-terminally processed

FTH

FTH1; FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15; FTH; FTHL6; OK/SW-cl.84; PIG15; Ferritin H subunit

FRIH_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of FTH. No significant cross-reactivity or interference between FTH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FTH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This FTH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FTH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: FTH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FTH antibody and an FTH-HRP conjugate. The assay sample and buffer are incubated together with FTH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FTH concentration since FTH from samples and FTH-HRP conjugate compete for the anti-FTH antibody binding site. Since the number of sites is limited, as more sites are occupied by FTH from the sample, fewer sites are left to bind FTH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FTH concentration in each sample is interpolated from this standard curve.

119594407

EAW74001.1

21,226 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Clathrin Derived Vesicle Budding Pathway (119545); Golgi Associated Vesicle Biogenesis Pathway (119546); Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (187191); Membrane Trafficking Pathway (106160); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Scavenging By Class A Receptors Pathway (833814); Transmembrane Transport Of Small Molecules Pathway (106572)

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family. Protein type: EC 1.16.3.1; Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: ferritin complex; mitochondrion; nucleus; cytosol. Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding. Biological Process: negative regulation of cell proliferation; receptor-mediated endocytosis; cellular iron ion homeostasis; negative regulation of fibroblast proliferation; immune response; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport; intracellular sequestering of iron ion. Disease: Hemochromatosis, Type 5

48-Strip-Wells

470 USD

96-Strip-Wells

675