catalog number :
MBS726236
products type :
ELISA Kit
products full name :
Human Scd8 ELISA Kit
products short name :
[Scd8]
products gene name :
[Scd8]
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive or Sandwich. Detection Range: 100 2500u/ml. Sensitivity: 1.0u/ml
other info2 :
Intended Uses: This sCD8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sCD8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: sCD8 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for sCD8. Standards or samples are then added to the microtiter plate wells and sCD8 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of sCD8 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for sCD8 are added to each well to "sandwich" the sCD8 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain sCD8 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sCD8 concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)
size5 :
5x96-Strip-Wells-(Competitive)
size6 :
5x96-Strip-Wells-(Sandwich)
size7 :
10x96-Strip-Wells-(Competitive)
size8 :
10x96-Strip-Wells-(Sandwich)