ELISA/assay

Human Thymosin beta 4 ELISA Kit

MBS726209

48-Strip-Wells

470 USD

ELISA Kit

Human Thymosin beta 4 ELISA Kit

Thymosin beta 4

Human Thymosin b 4 ELISA Kit

thymosin beta-4; Thymosin beta-4; thymosin beta-4; t beta-4; prothymosin beta-4; thymosin, beta 4, X chromosome; thymosin beta 4, X-linked; FxCleaved into the following chain:Hematopoietic system regulatory peptideAlternative name(s):Seraspenide

Tbeta4

Tb4

TMSB4X; TMSB4X; FX; TB4X; PTMB4; TMSB4; TB4X; THYB4; TMSB4; T beta-4

TYB4_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of T?4. No significant cross-reactivity or interference between T?4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between T?4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This T?4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human T?4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: T?4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for T?4. Standards or samples are then added to the microtiter plate wells and T?4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of T?4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for T?4 are added to each well to “sandwich” the T?4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain T?4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The T?4 concentration in each sample is interpolated from this standard curve.

11056061

NP_066932.1

NM_021109.3

5,053 Da

Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]

TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: Xq21.3-q22. Cellular Component: cytoskeleton; extracellular region. Molecular Function: actin monomer binding; protein binding. Biological Process: platelet activation; platelet degranulation; sequestering of actin monomers; actin filament organization; blood coagulation

48-Strip-Wells

470 USD

96-Strip-Wells

675