product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Bromodeoxyuridine ELISA Kit
catalog :
MBS726199
quantity :
96 Strip Wells
price :
675 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS726199
products type :
ELISA Kit
products full name :
Human Bromodeoxyuridine ELISA Kit
products short name :
Bromodeoxyuridine
products gene name :
BrDU
reactivity :
Human
storage stability :
Store all reagents at 2-8 degree C.
products description :
Intended Uses: This BRDU ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofBRDU in the sample, thisBRDU ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusBRDU concentration. The concentration ofBRDU in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This BRDU enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forBRDU. Standards or samples are then added to the microtiter plate wells andBRDU if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofBRDU present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forBRDU are added to each well to "sandwich" theBRDU immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containBRDU and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
size :
96 Strip Wells
price :
675 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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