catalog number :
MBS726162
products type :
ELISA Kit
products full name :
Rat Complement Component 2 ELISA Kit
products short name :
Complement Component 2
other names :
Complement component 2, isoform CRA_b; Complement component 2, isoform CRA_b; cDNA, FLJ92956, Homo sapiens complement component 2 (C2), mRNA
other gene names :
C2; hCG_43694
uniprot entry name :
Q5JP69_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of C2. No significant cross-reactivity or interference between C2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C2 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This C2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat C2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the assay: C2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for C2. Standards or samples are then added to the microtiter plate wells and C2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of C2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for C2 are added to each well to “sandwich” the C2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C2 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAQ07481.1
ncbi mol weight :
83,268 Da