ELISA/assay

Mouse Signal Transducer And Activator Of Transcription 3 ELISA Kit

MBS726159

48-Strip-Wells

470 USD

ELISA Kit

Mouse Signal Transducer And Activator Of Transcription 3 ELISA Kit

Signal Transducer And Activator Of Transcription 3

Stat3; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3; acute phase response factor; acute-phase response factor; signal transducer and activator of transcription 3; Acute-phase response factor

STAT3

Stat3; Stat3; Aprf; AW109958; 1110034C02Rik; Aprf

STAT3_MOUSE

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Signal Transduction

Intended Uses: This STAT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse STAT3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: STAT3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for STAT3. Standards or samples are then added to the microtiter plate wells and STAT3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of STAT3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for STAT3 are added to each well to "sandwich" the STAT3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain STAT3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STAT3 concentration in each sample is interpolated from this standard curve.

458706

AAA19452.1

87,967 Da

Acute Myeloid Leukemia Pathway (83310); Acute Myeloid Leukemia Pathway (529); Adipocytokine Signaling Pathway (83290); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198299); Androgen Receptor Signaling Pathway (198319); B Cell Receptor Signaling Pathway (198285); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864)

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described. Protein type: Transcription factor; DNA-binding; Nuclear receptor co-regulator; Motility/polarity/chemotaxis. Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; cytoplasm; plasma membrane; intracellular; nucleus. Molecular Function: protein dimerization activity; protein binding; ligand-dependent nuclear receptor activity; signal transducer activity; DNA binding; sequence-specific DNA binding; protein kinase binding; transcription factor activity; protein phosphatase binding; transcription factor binding; CCR5 chemokine receptor binding; glucocorticoid receptor binding. Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; glucose homeostasis; signal transduction; response to estradiol stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; astrocyte differentiation; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; eating behavior; cytokine and chemokine mediated signaling pathway; stem cell maintenance; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; cell proliferation; sexual reproduction; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; regulation of mitochondrial membrane permeability; eye photoreceptor cell differentiation; phosphorylation

48-Strip-Wells

470 USD

96-Strip-Wells

675