catalog number :
MBS726156
products type :
ELISA Kit
products full name :
Human Fascin ELISA Kit
products short name :
Fascin
other names :
fascin; Fascin; fascin; fascin homolog 1, actin-bundling protein; fascin actin-bundling protein 1<
products gene name :
FSCN
other gene names :
fscn1; fscn; fscn
uniprot entry name :
FASC_XENLA
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This FSCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FSCN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Signal Transduction
products description :
Principle of the Assay: FSCN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FSCN. Standards or samples are then added to the microtiter plate wells and FSCN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FSCN present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FSCN are added to each well to "sandwich" the FSCN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FSCN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSCN concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_001081581.1
ncbi gb acc num :
NM_001088112.1
ncbi mol weight :
53,023 Da
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)