catalog number :
MBS726125
products type :
ELISA Kit
products full name :
Mouse Parathormone 1-34 ELISA Kit
products short name :
Parathormone 1-34
other names :
pth; Peptidyl-tRNA hydrolase
products gene name :
PTH1-34
other gene names :
pth; Sca_0152; PTH
uniprot entry name :
PTH_STACT
specificity :
This assay has high sensitivity and excellent specificity for detection of PTH1-34. No significant cross-reactivity or interference between PTH1-34 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTH1-34 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This PTH1-34 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTH1-34. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: PTH1-34 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti- PTH1-34 antibody and an PTH1-34-HRP conjugate. The assay sample and buffer are incubated together with PTH1-34-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTH1-34 concentration since PTH1-34 from samples and PTH1-34-HRP conjugate compete for the anti- PTH1-34 antibody binding site. Since the number of sites is limited, as more sites are occupied by PTH1-34 from the sample, fewer sites are left to bind PTH1-34-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTH1-34 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAL27065.1
ncbi mol weight :
21,544 Da