product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Runt Related Transcription Factor 2 ELISA Kit
catalog :
MBS726105
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS726105
products type :
ELISA Kit
products full name :
Mouse Runt Related Transcription Factor 2 ELISA Kit
products short name :
Runt Related Transcription Factor 2
other names :
Runt-related transcription factor 2; Runt-related transcription factor 2
products gene name :
RUNX2
other gene names :
RUNX2
uniprot entry name :
Q5T802_HUMAN
reactivity :
Mouse
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Highest Standards: 25ng/mL. Concentration of Standards: Concentration of Standards: 0,1.0,2.5,5.0,10,25ng/mL
products description :
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This RUNX2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RUNX2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY RUNX2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RUNX2 antibody and an RUNX2-HRP conjugate. The assay sample and buffer are incubated together with RUNX2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RUNX2 concentration since RUNX2 from samples and RUNX2-HRP conjugate compete for the anti-RUNX2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RUNX2 from the sample, fewer sites are left to bind RUNX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RUNX2 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
55959066
ncbi acc num :
CAI13532.1
uniprot acc num :
Q5T802
ncbi mol weight :
64,439 Da
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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