ELISA/assay

Human Protein Kinase B Gamma ELISA Kit

MBS726076

48-Strip-Wells

470 USD

ELISA Kit

Human Protein Kinase B Gamma ELISA Kit

Protein Kinase B Gamma

protein kinase B gamma; RAC-gamma serine/threonine-protein kinase; RAC-gamma serine/threonine-protein kinase; PKB gamma; RAC-gamma serine/threonine protein kinase; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); v-akt murine thymoma viral oncogene homolog 3; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2

PKBg

AKT3; AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma; PKBG; PKB gamma

AKT3_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of PKBgamma. No significant cross-reactivity or interference between PKBgamma and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PKBgamma and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This PKBgamma ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PKBgamma. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: PKBgamma ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKBgamma antibody and an PKBgamma-HRP conjugate. The assay sample and buffer are incubated together with PKBgamma-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKBgamma concentration since PKBgamma from samples and PKBgamma-HRP conjugate compete for the anti-PKBgamma antibody binding site. Since the number of sites is limited, as more sites are occupied by PKBgamma from the sample, fewer sites are left to bind PKBgamma-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKBgamma concentration in each sample is interpolated from this standard curve.

4757579

AAD29089.1

54,032 Da

AKT Phosphorylates Targets In The Cytosol Pathway (106475); AKT Phosphorylates Targets In The Nucleus Pathway (106476); AKT-mediated Inactivation Of FOXO1A Pathway (106333); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160)

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Akt3: an AGC kinase that plays a critical role in controlling the balance between survival and apoptosis. Stimulated by platelet-derived growth factor, insulin, and insulin-like growth factor 1. Alternatively spliced isoforms have been described. Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, AGC; AGC group; AKT family. Chromosomal Location of Human Ortholog: 1q44. Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane. Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity. Biological Process: mitochondrial genome maintenance; signal transduction; protein amino acid phosphorylation. Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 2

48-Strip-Wells

470 USD

96-Strip-Wells

675