ELISA/assay

Human Perinuclear anti neutrophil cytoplasmic antibody ELISA Kit

MBS726069

96 Strip Wells

675 USD

ELISA Kit

Human Perinuclear anti neutrophil cytoplasmic antibody ELISA Kit

Perinuclear anti neutrophil cytoplasmic antibody

pANCA

Human

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Signal Transduction

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This pANCA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human pANCA. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY pANCA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-pANCA antibody and an pANCA-HRP conjugate. The assay sample and buffer are incubated together with pANCA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the pANCA concentration since pANCA from samples and pANCA-HRP conjugate compete for the anti-pANCA antibody binding site. Since the number of sites is limited, as more sites are occupied by pANCA from the sample, fewer sites are left to bind pANCA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pANCA concentration in each sample is interpolated from this standard curve.

96 Strip Wells

675 USD