ELISA/assay

Human beta tubulin ELISA Kit

MBS726060

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human beta tubulin ELISA Kit

beta tubulin

Human b tubulin ELISA Kit

TUBB, partial; Tubulin beta-2A chain; tubulin beta-2A chain; class IIa beta-tubulin; tubulin beta class IIa; tubulin, beta polypeptide 2; tubulin, beta 2A class IIa; Tubulin beta class IIa

TUBB

TUBB

TUBB2A; TUBB2A; TUBB; TUBB2; CDCBM5; dJ40E16.7; TUBB2

TBB2A_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This TUBbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TUBbeta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the Assay: TUBbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TUBbeta antibody and an TUBbeta-HRP conjugate. The assay sample and buffer are incubated together with TUBbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TUBbeta concentration since TUBbeta from samples and TUBbeta-HRP conjugate compete for the anti-TUBbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by TUBbeta from the sample, fewer sites are left to bind TUBbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TUBbeta concentration in each sample is interpolated from this standard curve.

49456871

CAG46756.1

Q13885

49,907 Da

Chaperonin-mediated Protein Folding Pathway (106242); Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway (106243); Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway (106245); Gap Junction Pathway (83072); Gap Junction Pathway (483); Metabolism Of Proteins Pathway (106230); Parkin-Ubiquitin Proteasomal System Pathway (700638); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (672459); Pathogenic Escherichia Coli Infection Pathway (516)

Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

TUBB2A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 6p25. Cellular Component: microtubule; cytoplasm; nucleus; vesicle. Molecular Function: GTPase activity; GTP binding; structural constituent of cytoskeleton. Biological Process: protein polymerization; de novo posttranslational protein folding; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process. Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 5

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675