ELISA/assay

Mouse beta Defensins ELISA Kit

MBS726058

48-Strip-Wells

470 USD

ELISA Kit

Mouse beta Defensins ELISA Kit

beta Defensins

Mouse b Defensins ELISA Kit

beta-defensin 103; Beta-defensin 103; beta-defensin 103; beta-defensin 3; defensin, beta 3; defensin, beta 103; defensin-like protein; defensin, beta 103A; Beta-defensin 3; BD-3; DEFB-3; HBD3; hBD-3; Defensin, beta 103; Defensin-like protein

BDF

BDF

DEFB103A; DEFB103A; BD-3; HBD3; HBP3; DEFB3; HBP-3; hBD-3; DEFB-3; DEFB103; BD3; DEFB103; DEFB3 DEFB103B ; BD-3; DEFB-3; HBD3; hBD-3

D103A_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of betaDEF. No significant cross-reactivity or interference between betaDEF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between betaDEF and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

Intended Uses: This betaDEF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse betaDEF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay betaDEF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-betaDEF antibody and an betaDEF-HRP conjugate. The assay sample and buffer are incubated together with betaDEF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the betaDEF concentration since betaDEF from samples and betaDEF-HRP conjugate compete for the anti-betaDEF antibody binding site. Since the number of sites is limited, as more sites are occupied by betaDEF from the sample, fewer sites are left to bind betaDEF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The betaDEF concentration in each sample is interpolated from this standard curve.

125656159

NP_001075020.1

NM_001081551.3

7,697 Da

Beta Defensins Pathway (530759); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, an antibiotic peptide which is induced by bacteria and interferon gamma, and which displays antimicrobial activity against S. aureus, S. pyogenes, P. aeruginosa, E. coli, and C. albicans. [provided by RefSeq, Oct 2014]

defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: Golgi lumen; extracellular region. Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria

48-Strip-Wells

470 USD

96-Strip-Wells

675