ELISA/assay

Mouse Anti-soluble liver antigen ELISA Kit

MBS726041

48-Strip-Wells

470 USD

ELISA Kit

Mouse Anti-soluble liver antigen ELISA Kit

Anti-soluble liver antigen

soluble liver antigen/liver pancreas antigen; O-phosphoseryl-tRNA(Sec) selenium transferase; O-phosphoseryl-tRNA(Sec) selenium transferase; O-phosphoseryl-tRNA(Sec) selenium transferase; SLA-p35; SLA/LP autoantigen; soluble liver antigen; liver-pancreas antigen; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein; soluble liver antigen/liver pancreas antigen; Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase; Liver-pancreas antigen; LP; SLA-p35; SLA/LP autoantigen; Selenocysteine synthase; Sec synthase; Selenocysteinyl-tRNA(Sec) synthase; Sep-tRNA:Sec-tRNA synthase; SepSecS; Soluble liver antigen; SLA; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein

SLA

SEPSECS; SEPSECS; LP; SLA; PCH2D; SLA/LP; TRNP48; LP; Sec synthase; SepSecS; SLA

SPCS_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This ASLAg ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ASLAg. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: ASLAg ELISA kit applies the competitive enzyme immunoassay technique utilizing soluble Liver Antigen and an ASLAg-HRP conjugate. The assay sample and buffer are incubated together with ASLAg-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASLAg concentration since ASLAg from samples and ASLAg-HRP conjugate compete for the soluble Liver Antigen binding site. Since the number of sites is limited, as more sites are occupied by ASLAg from the sample, fewer sites are left to bind ASLAg-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASLAg concentration in each sample is interpolated from this standard curve.

7652498

AAD33963.2

55,411 Da

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Selenium Metabolism And Selenoproteins Pathway (198846); Selenocompound Metabolism Pathway (82969); Selenocompound Metabolism Pathway (338)

The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]

SLA/LP: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis. Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D). PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures. Belongs to the SepSecS family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.9.1.2; RNA-binding; Transferase. Chromosomal Location of Human Ortholog: 4p15.2. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding; transferase activity, transferring selenium-containing groups; tRNA binding; pyridoxal phosphate binding. Biological Process: selenocysteine incorporation. Disease: Pontocerebellar Hypoplasia, Type 2d

48-Strip-Wells

470 USD

96-Strip-Wells

675