ELISA/assay

Mouse Urokinase-type Plasminogen Activator Receptor ELISA Kit

MBS726020

48-Strip-Wells

470 USD

ELISA Kit

Mouse Urokinase-type Plasminogen Activator Receptor ELISA Kit

Urokinase-type Plasminogen Activator Receptor

urokinase-type plasminogen activator receptor; Urokinase plasminogen activator surface receptor; urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; urokinase-type plasminogen activator (uPA) receptor; plasminogen activator, urokinase receptor; Monocyte activation antigen Mo3; CD_antigen: CD87

PLAUR/Upar

PLAUR; PLAUR; CD87; UPAR; URKR; U-PAR; MO3; UPAR; U-PAR; uPAR

UPAR_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL

Intended Uses: This uPAR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse uPAR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: uPAR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for uPAR Standards or samples are then added to the microtiter plate wells and uPAR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of uPAR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody specific for uPAR are added to each well to "sandwich" the uPAR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. Hie enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain uPAR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotonietrically at a wavelength of 450 nm A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The uPAR concentration in each sample is interpolated from this standard curve.

517198

AAB60333.1

32,016 Da

Arf6 Downstream Pathway (138038); Attachment Of GPI Anchor To UPAR Pathway (106239); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); FGF Signaling Pathway (137989); Hemostasis Pathway (106028); Metabolism Of Proteins Pathway (106230); Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway (106236)

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor; Receptor, misc.; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 19q13. Cellular Component: endoplasmic reticulum membrane; focal adhesion; extrinsic to membrane; integral to plasma membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane. Molecular Function: protein domain specific binding; protein binding; U-plasminogen activator receptor activity; enzyme binding; receptor activity; receptor binding. Biological Process: fibrinolysis; cellular protein metabolic process; regulation of proteolysis; attachment of GPI anchor to protein; C-terminal protein lipidation; post-translational protein modification; cell motility; chemotaxis; blood coagulation; signal transduction

48-Strip-Wells

470 USD

96-Strip-Wells

675