ELISA/assay

Mouse Prostaglandin E Synthase, Microsomal ELISA Kit

MBS726011

48-Strip-Wells

470 USD

ELISA Kit

Mouse Prostaglandin E Synthase, Microsomal ELISA Kit

Prostaglandin E Synthase, Microsomal

prostaglandin E synthase; Prostaglandin E synthase; prostaglandin E synthase; MGST1-like 1; p53-induced gene 12 protein; p53-induced apoptosis protein 12; glutathione S-transferase 1-like 1; microsomal prostaglandin E synthase 1; microsomal prostaglandin E synthase-1; tumor protein p53 inducible protein 12; microsomal glutathione S-transferase 1-like 1; prostaglandin E synthase; Microsomal glutathione S-transferase 1-like 1; MGST1-L1; Microsomal prostaglandin E synthase 1; MPGES-1; p53-induced gene 12 protein

PTGES

PTGES; PTGES; PGES; MPGES; PIG12; PP102; PP1294; MGST-IV; MGST1L1; TP53I12; mPGES-1; MGST1-L1; MGST1L1; MPGES1; PGES; PIG12; MGST1-L1; MPGES-1

PTGES_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of PGEsm. No significant cross-reactivity or interference between PGEsm and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGEsm and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This PGEsm ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PGEsm. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PGEsm ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGEsm antibody and an PGEsm-HRP conjugate. The assay sample and buffer are incubated together with PGEsm-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGEsm concentration since PGEsm from samples and PGEsm-HRP conjugate compete for the anti-PGEsm antibody binding site. Since the number of sites is limited, as more sites are occupied by PGEsm from the sample, fewer sites are left to bind PGEsm-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGEsm concentration in each sample is interpolated from this standard curve.

4758910

NP_004869.1

NM_004878.4

17,102 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Synthesis Of Prostaglandins (PG) And Thromboxanes (TX) Pathway (730333)

The protein encoded by this gene is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53 induced apoptosis. Knockout studies in mice suggest that this gene may contribute to the pathogenesis of collagen-induced arthritis and mediate acute pain during inflammatory responses. [provided by RefSeq, Jul 2008]

PTGES: Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Belongs to the MAPEG family. Protein type: Lipid Metabolism - arachidonic acid; Nuclear envelope; Membrane protein, integral; Isomerase; EC 5.3.99.3; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 9q34.3. Cellular Component: endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; nuclear envelope lumen; integral to membrane. Molecular Function: glutathione binding; prostaglandin-E synthase activity. Biological Process: negative regulation of cell proliferation; chronic inflammatory response; response to retinoic acid; response to cytokine stimulus; cyclooxygenase pathway; arachidonic acid metabolic process; response to lipopolysaccharide; signal transduction; prostaglandin biosynthetic process; response to calcium ion; response to organic cyclic substance; acute inflammatory response; prostaglandin metabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675