ELISA/assay

Mouse Cyclophosphamide ELISA Kit

MBS725997

48-Strip-Wells

470 USD

ELISA Kit

Mouse Cyclophosphamide ELISA Kit

Cyclophosphamide

CTX

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich

For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This BG CP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CP. Standards or samples are then added to the microtiter plate wells and CP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CP are added to each well to "sandwich" the CP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CP concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675