ELISA/assay

Mouse Annexin A2 ELISA Kit

MBS725920

48-Strip-Wells

470 USD

ELISA Kit

Mouse Annexin A2 ELISA Kit

Annexin A2

annexin A2 isoform 2; Annexin A2; annexin A2; annexin-2; protein I; annexin II; lipocortin II; chromobindin 8; chromobindin-8; calpactin I heavy chain; calpactin-1 heavy chain; calpactin I heavy polypeptide; placental anticoagulant protein IV; epididymis secretory protein Li 270; annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin-8; Lipocortin II; Placental anticoagulant protein IV; PAP-IV; Protein I; p36

ANXA2

ANXA2; ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270; ANX2; ANX2L4; CAL1H; LPC2D; PAP-IV

ANXA2_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Cell Biology

Intended Uses: This ANXA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ANXA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ANXA2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ANXA2. Standards or samples are then added to the microtiter plate wells and ANXA2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ANXA2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ANXA2 are added to each well to "sandwich" the ANXA2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ANXA2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA2 concentration in each sample is interpolated from this standard curve.

209862831

NP_001129487.1

NM_001136015.2

40,411 Da

Dissolution Of Fibrin Clot Pathway (106061); Hemostasis Pathway (106028); Prostaglandin Synthesis And Regulation Pathway (198912)

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion. Protein type: Calcium-binding; Motility/polarity/chemotaxis; Lipid-binding. Chromosomal Location of Human Ortholog: 15q22.2. Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; protein complex; lysosomal membrane; late endosome membrane; early endosome; lipid particle; cell cortex; lipid raft; ruffle; extracellular matrix; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; basement membrane; midbody; nucleus; vesicle; endosome; sarcolemma. Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; cytoskeletal protein binding; calcium ion binding; calcium-dependent protein binding; Rab GTPase binding. Biological Process: positive regulation of fibroblast proliferation; fibrinolysis; collagen fibril organization; negative regulation of catalytic activity; protein heterotetramerization; positive regulation of vesicle fusion; positive regulation of protein amino acid phosphorylation; angiogenesis; positive regulation of binding; lipid raft formation; body fluid secretion; membrane budding

48-Strip-Wells

470 USD

96-Strip-Wells

675